Production of wax esters via microbial oil synthesis from food industry waste and by-product streams. (December 2017)

• Record Type: Journal Article
• Authors: Papadaki, Aikaterini; Mallouchos, Athanasios; Efthymiou, Maria-Nefeli; Gardeli, Chryssavgi; Kopsahelis, Nikolaos; Aguieiras, Erika C.G.; Freire, Denise M.G.; Papanikolaou, Seraphim; Koutinas, Apostolis A.
• Is Part Of: Bioresource technology. Volume 245:Part A(2017)
• Page Start: 274
• Page End: 282
• Publication Date: 2017-12
• Subjects: Wax esters -- Microbial oil -- Food processing waste and by-products -- Lipase -- Oleochemicals; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.08.004
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Biorefining of food wastes for bio-based wax esters production. Microbial oil was converted to wax esters through solvent-free enzymatic catalysis. Microbial wax esters of behenyl alcohol achieved high conversion yield of 87.3%. Behenyl wax ester conversion yield was more than 80% after 3 repeated reactions. The microbial bio-based wax esters presented similar properties to natural waxes. Abstract: The production of wax esters using microbial oils was demonstrated in this study. Microbial oils produced from food waste and by-product streams by three oleaginous yeasts were converted into wax esters via enzymatic catalysis. Palm oil was initially used to evaluate the influence of temperature and enzyme activity on wax ester synthesis catalysed by Novozyme 435 and Lipozyme lipases using cetyl, oleyl and behenyl alcohols. The highest conversion yields (up to 79.6%) were achieved using 4 U/g of Novozyme 435 at 70 °C. Transesterification of microbial oils to behenyl and cetyl esters was achieved at conversion yields up to 87.3% and 69.1%, respectively. Novozyme 435 was efficiently reused for six and three cycles during palm esters and microbial esters synthesis, respectively. The physicochemical properties of microbial oil derived behenyl esters were comparable to natural waxes. Wax esters from microbial oils have potential applications in cosmetics, chemical and food industries.