A two-step optimization strategy for 2nd generation ethanol production using softwood hemicellulosic hydrolysate as fermentation substrate. (November 2017)

• Record Type: Journal Article
• Authors: Boboescu, Iulian-Zoltan; Gélinas, Malorie; Beigbeder, Jean-Baptiste; Lavoie, Jean-Michel
• Is Part Of: Bioresource technology. Volume 244:Part 1(2017)
• Page Start: 708
• Page End: 716
• Publication Date: 2017-11
• Subjects: DE design of experiments -- CCD central-composite design -- RSM response surface methodology -- IF influencing factors -- OF objective functions; Hemicellulosic ethanol -- Softwood residue -- Steam-explosion pretreatment -- Central-composite design -- Response-surface methodology; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.07.107
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: 8 factors concerning yeast, nutrients and process parameters were investigated. Yeast inoculum, pH and nutrient availability were determined the most significant. Process modeling was performed and optimization scenarios were predicted. A maximum of 74.27% of hemicellulosic ethanol yield was produced. Optimization confirmations and bioreactor scale-up were performed. Abstract: Ethanol production using waste biomass represents a very attractive approach. However, there are considerable challenges preventing a wide distribution of these novel technologies. Thus, a fractional-factorial screening of process variables and Saccharomyces cerevisiae yeast inoculum conditions was performed using a synthetic fermentation media. Subsequently, a response-surface methodology was developed for maximizing ethanol yields using a hemicellulosic solution generated through the chemical hydrolysis of steam treatment broth obtained from residual softwood biomass. In addition, nutrient supplementation using starch-based ethanol production by-products was investigated. An ethanol yield of 74.27% of the theoretical maximum was observed for an initial concentration of 65.17 g/L total monomeric sugars. The two-step experimental strategy used in this work represents the first successful attempt to developed and use a model to make predictions regarding the optimal ethanol production using both softwood feedstock residues as well as 1st generation ethanol production by-products.