Exploration of a mechanism for the production of highly unsaturated fatty acids in Scenedesmus sp. at low temperature grown on oil crop residue based medium. (November 2017)

• Record Type: Journal Article
• Authors: Lu, Qian; Li, Jun; Wang, Jinghan; Li, Kun; Li, Jingjing; Han, Pei; Chen, Paul; Zhou, Wenguang
• Is Part Of: Bioresource technology. Volume 244:Part 1(2017)
• Page Start: 542
• Page End: 551
• Publication Date: 2017-11
• Subjects: Oil crop residue -- Scenedesmus sp. -- Carbon allocation -- Unsaturated fatty acids -- Mechanisms; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.08.005
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Diluted oil crop residue is a good medium for Scenedesmus sp. cultivation. Low temperature improves accumulation of eicosapentaenoic acid. Highly unsaturated fatty acids are synthesized in priority at early stage. Algal cells adjust carbon flux distribution based on self-protection mechanism. Abstract: The ability of algae to produce lipids comprising of unsaturated fatty acids varies with strains and culture conditions. This study investigates the effect of temperature on the production of unsaturated fatty acids in Scenedesmus sp. grown on oil crop residue based medium. At low temperature (10 °C), synthesis of lipids compromising of high contents of unsaturated fatty acids took place primarily in the early stage while protein accumulation mainly occurred in the late stage. This stepwise lipid-protein synthesis process was found to be associated with the contents of acetyl-CoA and α-KG in the algal cells. A mechanism was proposed and tested through simulation experiments which quantified the carbon flux allocation in algal cells at different cultivation stages. It is concluded that low culture temperature such as 10 °C is suitable for the production of lipids comprising of unsaturated fatty acids.