Continuous feeding of spent ammonium sulphite liquor improves the production and saccharification performance of cellulase by Penicillium oxalicum. (December 2017)

• Record Type: Journal Article
• Authors: Han, Xiaolong; Liu, Guodong; Song, Wenxia; Qin, Yuqi; Qu, Yinbo
• Is Part Of: Bioresource technology. Volume 245:Part A(2017)
• Page Start: 984
• Page End: 992
• Publication Date: 2017-12
• Subjects: Cellulase -- Penicillium oxalicum -- Spent ammonium sulphite liquor -- Fed-batch fermentation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.09.042
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Fermentation with continuous feeding SASL improved cellulase production. The maximum FPase reached 17.66 U/mL using a multi-stage feeding strategy. Comparative proteomic analysis showed that SASL induced cellulases synthesis. The SASL-induced cellulase could hydrolyze ammonium sulphite pulp efficiently. Abstract: Spent ammonium sulphite liquor (SASL) is the main effluent from the ammonium sulphite pulping process, and contains amounts of lignocellulosic oligomers, monosaccharides and ammonium salts. The effect of continuous SASL-feeding on cellulase production by Penicillium oxalicum was studied. With a rate-varying feeding strategy, the maximal filter paper enzyme (FPase) activity reached 17.66 U/mL at 144 h, and the specific FPase activity increased from 1.74 U/mg (without SASL) to 2.40 U/mg. Accordingly, the glucan hydrolysis conversion of delignified corn cob residue by the enzymes from continuous SASL-feeding fermentation was significantly higher than that without SASL at equal protein loadings. Comparative proteomic analysis demonstrated that the proteins involved in lignocellulose degradation were specifically up-regulated in the crude cellulase with SASL-feeding. The obtained crude enzyme was efficient in the hydrolysis of pulping products, with a glucan conversion of 81.87% achieved after 72 h saccharification of ammonium sulphite pulp.