Development and evaluation of consolidated bioprocessing yeast for ethanol production from ionic liquid-pretreated bagasse. (December 2017)

• Record Type: Journal Article
• Authors: Amoah, Jerome; Ishizue, Naoya; Ishizaki, Miki; Yasuda, Misa; Takahashi, Kenji; Ninomiya, Kazuaki; Yamada, Ryosuke; Kondo, Akihiko; Ogino, Chiaki
• Is Part Of: Bioresource technology. Volume 245:Part B(2017)
• Page Start: 1413
• Page End: 1420
• Publication Date: 2017-12
• Subjects: Bioethanol -- Lignocellulose -- Cocktail δ-integration -- Cell surface display -- Enzyme activity; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.05.171
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Cocktail δ-integration was used for the introduction of cellulase genes. Various consolidated bioprocess (CBP) yeast strains resulted. Ethanol production by CBP strains from different biomasses varied. A CBP yeast strain produced 91.2% ethanol from ionic liquid-pretreated bagasse. Copy numbers of integrated genes varied from transformant to transformant. Abstract: This work aimed to study the use of consolidated bioprocess (CBP) yeast expressing five cellulase genes ( BGL, XYNII, EGII, CBHI and CBHII ) for ethanol production from ionic liquid-pretreated bagasse and Laubholz unbleached Kraft pulp (LUKP). A proposed screening method shows that the optimal cellulase ratio varies for each biomass substrate, and thus it is essential to breed CBP yeast having optimal cellulase-displaying ratio for the target biomass. CBP yeast specialized towards bagasse produced 0.93 g/l ethanol whiles that for LUKP produced 0.71 g/l ethanol, which is approximately 4 and 2-fold, respectively, higher than that of the wild type. The cell-surface displayed enzymes synergistically contributed to the degradation of the biomass. The developed CBP yeast is a potential cheap source for consolidated bioprocessing of ethanol and the proposed screening method can be used for matching CBP yeast to a target biomass.