Arabidopsis NITROGEN LIMITATION ADAPTATION regulates ORE1 homeostasis during senescence induced by nitrogen deficiency. (November 2018)

• Record Type: Journal Article
• Authors: Park, Bong; Yao, Tao; Seo, Jun; Wong, Eriko; Mitsuda, Nobutaka; Huang, Chung-Hao; Chua, Nam-Hai
• Is Part Of: Nature plants. Volume 4:Number 11(2018)
• Page Start: 898
• Page End: 903
• Publication Date: 2018-11
• Subjects: Plants -- Periodicals; 580.5; 580 21
• DOI: 10.1038/s41477-018-0269-8
• Languages: English
• ISSNs: 2055-0278

Abstract

Abstract Nitrogen is an important macronutrient in plants and its deficiency induces rapid leaf senescence. Two genes, ORE1 and NITROGEN LIMITATION ADAPTATION (NLA), have been implicated in regulating the senescence process but their relationship is unclear1, 2 . Here, we show that nla and pho2 (also known as ubc24) plants develop rapid leaf senescence under nitrogen-starvation condition, whereas ore1 and nla/ore1 and pho2 (ubc24)/ore1 plants stay green. These results suggest that ORE1 acts downstream of NLA and PHO2 (UBC24). NLA interacts with ORE1 in the nucleus and regulates its stability through polyubiquitination using PHO2 (UBC24) as the E2 conjugase. Our findings identified ORE1 as a downstream target of NLA/PHO2 (UBC24) and showed that post-translational regulation of ORE1 levels determines leaf senescence during nitrogen deficiency. Nitrogen deficiency can lead to severe senescence and harvest losses. The regulatory mechanism depends on the NAC-domain-containing transcription factor ORE1. Now researchers have elucidated the critical post-translational pathway that regulates the protein levels of ORE1.