Nano-formulated curcumin (Lipodisq™) modulates the local inflammatory response, reduces glial scar and preserves the white matter after spinal cord injury in rats. (1st September 2019)

• Record Type: Journal Article
• Authors: Krupa, Petr; Svobodova, Barbora; Dubisova, Jana; Kubinova, Sarka; Jendelova, Pavla; Machova Urdzikova, Lucia
• Is Part Of: Neuropharmacology. Volume 155(2019)
• Page Start: 54
• Page End: 64
• Publication Date: 2019-09-01
• Subjects: Spinal cord injury -- Nanocurcumin -- Inflammatory response -- Neuroregeneration -- Astrogliosis -- Axonal growth; Neuropsychopharmacology -- Periodicals; Autonomic Agents -- Periodicals; Neuropsychopharmacologie -- Périodiques; Neuropsychopharmacology; Periodicals; Electronic journals; 615.78
• DOI: 10.1016/j.neuropharm.2019.05.018
• Languages: English
• ISSNs: 0028-3908

Abstract

Abstract: A highly water soluble, nano-formulated curcumin was used for the treatment of the experimental model of spinal cord injury (SCI) in rats. Nanocurcumin and a vehicle nanocarrier as a control, were delivered both locally, immediately after the spinal cord injury, and intraperitoneally during the 4 consecutive weeks after SCI. The efficacy of the treatment was assessed using behavioral tests, which were performed during the experiment, weekly for 9 weeks. The behavioral tests (BBB, flat beam test, rotarod, motoRater) revealed a significant improvement in the nanocurcumin treated group, compared to the nanocarrier control. An immunohistochemical analysis of the spinal cord tissue was performed at the end of the experiment and this proved a significant preservation of the white matter tissue, a reduced area of glial scaring and a higher amount of newly sprouted axons in the nanocurcumin treated group. The expression of endogenous genes ( Sort1, Fgf2, Irf5, Mrc1, Olig2, Casp3, Gap43, Gfap, Vegf, Nfkβ) and interleukins (IL-1β, TNF-α, IL-6, IL-12, CCL-5, IL-11, IL-10, IL-13) was evaluated by qPCR and showed changes in the expression of the inflammatory cytokines in the first two weeks after SCI. Highlights: Treatment by nanocurcumin improved functional outcome in rats after SCI. Nanocurcumin preserved damaged white matter and downsized the glial scar. Nanocurcumin treatment modulated the early inflammatory response.