6-Gingerol abates benzo[a]pyrene-induced colonic injury via suppression of oxido-inflammatory stress responses in BALB/c mice. (1st July 2019)

• Record Type: Journal Article
• Authors: Ajayi, Babajide O.; Adedara, Isaac A.; Farombi, Ebenezer O.
• Is Part Of: Chemico-biological interactions. Volume 307(2019)
• Page Start: 1
• Page End: 7
• Publication Date: 2019-07-01
• Subjects: [6]-gingerol -- benzo[a]pyrene -- Colon -- Inflammation -- Epoxide hydrolase; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2019.04.026
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Exposure to benzo[a]pyrene (BaP), the most toxic polycyclic aromatic hydrocarbon and a procarcinogen, is a global health concern which necessitates preventive measures. [6]-Gingerol (6-G), the most pharmacologically active constituent of ginger has been reported to promote gut health in various experimental settings. This study investigated the role of 6-G in BaP-induced colonic oxidative and inflammatory stress responses in mice. Experimental mice were randomly assigned into five groups of eight mice each and were orally gavage with BaP (125 mg/kg) singly or in combination with 6-G at 50 and 100 mg/kg for 14 consecutive days. Following sacrifice, the colonic activities of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), myeloperoxidase (MPO) as well as levels of glutathione (GSH), nitrites and lipid peroxidation (LPO) were assessed spectrophotometrically. Moreover, colonic concentration of epoxide hydrolase (EPXH), tumor necrosis factor alpha (TNF-α), interleukin-1 β (IL-1β), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) were assessed using ELISA. Administration of 6-G augmented BaP detoxification and colonic antioxidant status by increasing the EPXH, GST, SOD and CAT activities, GSH level with concomitant decrease in MDA level when compared with BaP alone group. In addition, 6-G suppressed BaP-induced colonic inflammation by decreasing MPO activity as well as nitrites, TNF-α, IL-1β, COX-2 and iNOS levels when compared with BaP alone group. In conclusion, 6-G protected against a decrease in colonic epoxide detoxifying enzymes and antioxidant defense mechanisms caused by BaP. Highlights: Benzo[a]pyrene (BaP) induced colonic injury in mice. [6]-Gingerol (6-G) improved colonic antioxidant status in BaP-treated mice. 6-G suppressed BaP-induced colonic TNF-α, IL-1β, COX-2 and iNOS concentration. 6-G improved colonic epoxide detoxifying enzymes, EPXH and GST, in BaP-treated mice. 6-G ameliorated BaP-induced colonic oxido-inflammatory stress in mice.