An integrated impedance biosensor platform for detection of pathogens in poultry products. Issue 1 (December 2018)

• Record Type: Journal Article
• Authors: Liu, Jiayu; Jasim, Ibrahem; Abdullah, Amjed; Shen, Zhenyu; Zhao, Lu; El-Dweik, Majed; Zhang, Shuping; Almasri, Mahmoud
• Is Part Of: Scientific reports. Volume 8:Issue 1(2018)
• Page Start: 1
• Page End: 10
• Publication Date: 2018-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-018-33972-0
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract This paper presents an impedance-based biosensor for rapid and simultaneous detection ofSalmonella serotypes B, D, and E with very low concentration. The biosensor consists of a focusing region, and three detection regions. The cells focusing was achieved using a ramp down electroplated vertical electrode pair along with tilted thin film finger pairs that generate p-DEP forces to focus and concentrate the bacterial cells into the center of the microchannel, and direct them toward the detection region. The detection regions consist of three interdigitated electrode arrays (IDEA), each with 20 pairs of finger coated with a mixture of anti-Salmonella antibody and crosslinker to enhance the adhesion to IDEA. The impedance changes as the targetSalmonella binds to the antibody. The biosensor has showed excellent performance as proven by the detection of a singleSalmonella serotype B, and simultaneous detection of twoSalmonella serotypes B and D with a limit of detection (LOD) of 8 Cells/ml in ready-to-eat turkey samples, the addition of focusing capability improved the measured signal by a factor of between 4–4.5, the total detection time of 45 minutes, selectivity of the sensor on different types of bacterial cells, and the ability to distinguish between dead and live cells.