Single Layer Centrifugation with Androcoll-P Can Be Scaled-Up to Process Larger Volumes of Boar Semen. (8th November 2010)

• Record Type: Journal Article
• Authors: van Wienen, Marjet; Johannisson, Anders; Wallgren, Margareta; Parlevliet, Joyce; Morrell, Jane M.
• Other Names: Gamble R. Academic Editor.; Yin Y. Academic Editor.
• Is Part Of: ISRN veterinary science. Volume 2011(2011)
• Publication Date: 2010-11-08
• Subjects: Veterinary medicine -- Periodicals; Veterinary Medicine; Veterinary medicine; Periodical; Periodicals; Electronic journals; Fulltext; Internet Resources; Periodicals; 636.089
• DOI: 10.5402/2011/548385
• Languages: English
• ISSNs: 2090-4452

Abstract

Abstract : The objective of this study was to scale-up the procedure for Single Layer Centrifugation (SLC) through Androcoll TM -P, as a preliminary step towords processing the whole ejaculate. The first experiment compared Single Layer Centrifugation using 4.5 mL and 15 mL extended ejaculate (SLC-4.5 and SLC-15, resp.), assessing sperm quality by objective motility analysis, morphology, viability, and the production of reactive oxygen species (ROS). In the second experiment, SLC-4.5 was compared to Single Layer Centrifugation with 25 mL extended ejaculate (SLC-25) using motility analysis and morphology. In both experiments, normal morphology and linear motility were significantly higher in the SLC-selected samples than in the uncentrifuged controls (P < .001 ), whereas total motility and membrane integrity were unchanged. Although ROS production was higher in the SLC-selected samples than in the controls (P < .01 ), this might have been due to the presence of antioxidants in seminal plasma in the latter. In conclusion, there was no difference in sperm quality between SLC-4.5 and SLC-15 samples, or between SLC-4.5 and SLC-25 samples, indicating that the SLC method can be scaled-up successfully.