Time Exposure Period of Bovine Oocytes to Sperm in Relation to Embryo Development Rate and Quality. (15th February 2011)

• Record Type: Journal Article
• Authors: Berland, Marco; Frei, Mario; Peralta, Oscar; Ratto, Marcelo
• Other Names: Gamble R. Academic Editor.
• Is Part Of: ISRN veterinary science. Volume 2011(2011)
• Publication Date: 2011-02-15
• Subjects: Veterinary medicine -- Periodicals; Veterinary Medicine; Veterinary medicine; Periodical; Periodicals; Electronic journals; Fulltext; Internet Resources; Periodicals; 636.089
• DOI: 10.5402/2011/257627
• Languages: English
• ISSNs: 2090-4452

Abstract

Abstract : The objective of the study was to determine the effect of different bovine gamete coincubation times on fertilization and embryo development performance. In vitro matured COCs were co-incubated with sperm at a concentration of 1.5 × 10 6 spermatozoa/ml in TALP medium for 3 hours (T 3, n = 362 ), 6 hours (T 6, n = 358 ), or 18 hours (T 18, n = 350 ). At the end of the coincubation period COCs from times 3 and 6 groups were post-incubated in a new well of fertilization medium without sperm for additional 15 and 12 h, respectively. Cumulus Oocyte Complexes from the T 18 were co-incubated with the sperm suspension for 18 hours. Presumptive zygotes were cultured for 9 days and embryo development was evaluated on days 2, 8, and 9. Thirty blastocysts from each group were stained and total number of nuclei was recorded. The mean (± SEM) percentages of zygotes to develop into ≥2 cell stage were 71.9 ± 5.0; 72.5 ± 5.3 and 81.2 ± 6.1 % for T 3, 6, and 18, respectively, on day 2 and they did not differ (P = . 3 ) among groups. The mean percentage of blastocysts developed on day 8 (25.6 ± 2.8; 24.2 ± 3.3; 28.4 ± 4.2 % for T 3, 6, and 18, resp.) did not differ (P = . 4 ) among groups. The total number of embryonic nuclei was greater (P < . 05 ) for the blastocysts produced from the shortest co-incubation time (T 3).