Channel from bacterial virus T7 DNA packaging motor for the differentiation of peptides composed of a mixture of acidic and basic amino acids. (September 2019)

• Record Type: Journal Article
• Authors: Ji, Zhouxiang; Guo, Peixuan
• Is Part Of: Biomaterials. Volume 214(2019)
• Publication Date: 2019-09
• Subjects: Bacteriophage DNA packaging motor -- T7 channel -- Nanopore -- Analysis of protein sequence -- Detection of protein mutation -- Biomotor; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2019.119222
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Protein mutations can result in dysfunctional cell signaling pathways; therefore it is of significance to develop a robust platform for the detection of protein mutations. Here, we report that the channel of bacterial virus T7 DNA packaging motor is able to discriminate peptides containing a mixture of acidic (negatively charged) and basic (positively charged) amino acids. Peptides were differentiated based on their current signatures created by their unique charge compositions. In combination with protease digestion, peptides with the locational differences of single amino acid were also identified. The results suggest that the T7 motor channel has the potential for peptide differentiation, mutation verification, and analysis of protein sequence. Graphical abstract: Image 1052