Trifunctional integrated DNA-based universal sensing platform for detection of diverse biomolecules in one-pot isothermal exponential amplification mode. Issue 53 (14th June 2019)

• Record Type: Journal Article
• Authors: Cui, Yun-Xi; Feng, Xue-Nan; Li, Xiao-Yu; Zhang, Yu-Peng; Tang, An-Na; Kong, De-Ming
• Is Part Of: Chemical communications. Volume 55:Issue 53(2019)
• Page Start: 7603
• Page End: 7606
• Publication Date: 2019-06-14
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc03758f
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Template constructed with only a short single-stranded DNA is necessary; it is first recognized by the target enzyme, then multiple amplification cycles are initiated, achieving ultra-high sensitivity by a one-pot isothermal reaction. Abstract : A biosensor with all the advantages of ultra-high sensitivity, easy operation, straightforward signal output and universal applicability is introduced. The biosensor was demonstrated to work well in the detection of polynucleotide kinase and DAM methyltransferase, thus providing a powerful tool for clinical diagnosis, drug screening and disease therapeutic assay.