MTOR/miR-145-regulated exosomal GOLM1 promotes hepatocellular carcinoma through augmented GSK-3β/MMPs. (20th May 2019)

• Record Type: Journal Article
• Authors: Gai, Xiaochen; Tang, Bufu; Liu, Fangming; Wu, Yuting; Wang, Fang; Jing, Yanling; Huang, Fuqiang; Jin, Di; Wang, Ling; Zhang, Hongbing
• Is Part Of: Journal of genetics and genomics. Volume 46:Number 5(2019)
• Page Start: 235
• Page End: 245
• Publication Date: 2019-05-20
• Subjects: GOLM1 -- miR-145 -- mTOR -- Exosome -- Hepatocellular carcinoma; CDS coding sequences -- DMEM Dulbecco's modified Eagle's medium -- FBS fetal bovine serum -- GOLM1/GP73 Golgi membrane protein 1 -- shV-exo GOLM1-enriched exosome -- shGOLM1-exo GOLM1-poor exosome -- HCC hepatocellular carcinoma -- H&E hematoxylin and eosin -- mTOR mammalian/mechanistic target of rapamycin -- MEFs mouse embryonic fibroblasts -- PBS phosphate buffer solution -- qRT-PCR quantitative RT-PCR -- SD standard deviation -- TSC Tuberous Sclerosis Complex; Genetics -- Periodicals; Genomics -- Periodicals; 576.505
• DOI: 10.1016/j.jgg.2019.03.013
• Languages: English
• ISSNs: 1673-8527

Abstract

Abstract: Golgi membrane protein 1 (GOLM1/GP73) is a serum marker of hepatocellular carcinoma (HCC). We have previously shown that mTOR promoted tumorigenesis of HCC through stimulating GOLM1 expression. In this study, we demonstrated that the mammalian target of rapamycin (mTOR) was a negative regulator of microRNA-145 ( miR-145 ) expression. miR-145 inhibited GOLM1 expression by targeting a coding sequence of GOLM1 gene. GOLM1 and miR-145 were inversely correlated in human HCC tissues. GOLM1-enriched exosomes activated the glycogen synthase kinase-3β/matrix metalloproteinases (GSK-3β/MMPs) signaling axis of recipient cells and accelerated cell proliferation and migration. In contrast, miR-145 suppressed tumorigenesis and metastasis. We suggest that mTOR/ miR-145 /GOLM1 signaling pathway should be targeted for HCC treatment.