Phenotypic Characterization and Genotypic Subtyping of Salmonella enterica Serovars Enteritidis and Gallinarum Isolated from Human and Poultry-Related Samples. Issue 3 (3rd July 2018)

• Record Type: Journal Article
• Authors: Boukoucha, Mourad; Menasria, Taha; Bouguerra, Nadia
• Is Part Of: Food biotechnology. Volume 32:Issue 3(2018)
• Page Start: 206
• Page End: 221
• Publication Date: 2018-07-03
• Subjects: Salmonella -- S. Enteritidis -- S. Gallinarum -- poultry product -- antimicrobial resistance -- MLST -- MLVA; Food -- Biotechnology -- Periodicals; Biotechnology -- Periodicals; Food Technology -- Periodicals; Food-Processing Industry -- Periodicals
• DOI: 10.1080/08905436.2018.1507912
• Languages: English
• ISSNs: 0890-5436

Abstract

ABSTRACT: This study aimed to assess the serotypes, antibiotic susceptibility, and molecular subtyping of Salmonella enterica isolated from food products and human patients with gastroenteritis. A total of 59 isolates were investigated, and the results revealed a predominance of S . Enteritidis with 57.63% (34/59) S . Gallinarum held second with 15.62% (5/32) of total food borne. While, isolates from humans showed 18.51% (5/27) of S . Typhimurium. High level of resistance to nalidixic acid was noted among food strains and 35.29% of human isolates were found to be multidrug resistant. Eight representative isolates were subtyping using three molecular approaches, ribotyping, Multilocus sequence typing (MLST) and Multiple-locus variable number tandem repeat analysis (MLVA). MLST showed three sequence types corresponding to two clonal complexes, (ST-78, CC-4) for S . Gallinarum, (ST-11, CC-4) for S . Enteritidis and (S-367, CC-37) for S . Cerro. While, MLVA generated six different profiles targeting nine loci for S . Enteritidis and S . Gallinarum.