A murine model of wheat versus potato allergy: Patatin and 53kDa protein are the potential allergen from potato. (September 2018)

• Record Type: Journal Article
• Authors: Ansari, Ibtessam Tahir; Mu, Taihua
• Is Part Of: Molecular immunology. Volume 101(2018:Sep.)
• Page Start: 284
• Page End: 293
• Publication Date: 2018-09
• Subjects: P1 protease inhibitors -- P2 patatin -- WG wheat gluten -- PBS phosphate buffer saline -- PP potato protein -- TBS Tris buffer saline -- TBST Tris buffer saline with tween 20; Potato protein -- Allergy -- Cytokines -- IgE -- Vascular permeability; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2018.07.012
• Languages: English
• ISSNs: 0161-5890

Abstract

Graphical abstract: Highlights: A BALB/c mice model was established to analyze the allergen in potato protein. Patatin and protease inhibitors of potato protein were purified and characterized. Potato protein had a relative lower antigen-specific immunoglobulin E (IgE) level. Patatin exhibited higher hypersensitivity reactions than protein and protease inhibitors. Immuno-blot showed that 53 kDa factions in potato protein had IgE-binding capacity. Abstract: Wheat allergy is the most common around the world as gluten is the potential allergen. People diagnosed with wheat allergy were mainly substitute with other novel food such as potato though it is also being reported for allergenic manifestations. Thus there is an increasing demand for developing a BALB/c mice model to empathize the allergic properties of potato protein and its fractions. Purified potato protein showed lower IgE-binding capacity (474.39 ± 0.6 ng/mL) even in higher concentration (30 mg/mL) compared to wheat gluten (1418.28 ± 0.17 ng/mL, 5mg/mL). Immediate active cutaneous hypersensitivity reaction, vascular leakage, intestinal permeability and lung's inflammatory cell infiltration was also ascertained comparatively lower in potato protein than wheat gluten. Furthermore, patatin (43 kDa) and protease inhibitors (∼21 kDa) were purified and separated, and patatin exhibited higher hypersensitivity score than that of protease inhibitors. Immuno-detection assays indicated that patatin and 53 kDa protein in potato protein showed specific Ig-E binding capacity, and 53 kDa was adenosyl homocysteinase identified by LC–MS/MS.