Chemical constituents from the rare mushroom Calvatia nipponica inhibit the promotion of angiogenesis in HUVECs. Issue 17 (1st September 2017)

• Record Type: Journal Article
• Authors: Lee, Seulah; Park, Jun Yeon; Lee, Dahae; Seok, Soonja; Kwon, Yeon Jung; Jang, Tae Su; Kang, Ki Sung; Kim, Ki Hyun
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 27:Issue 17(2017)
• Page Start: 4122
• Page End: 4127
• Publication Date: 2017-09-01
• Subjects: Calvatia nipponica -- Agaricaceae -- Alkaloid -- Angiogenesis -- VEGF; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2017.07.036
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: Calvatia species, also known as puffball mushrooms, are common sources of food worldwide. Calvatia nipponica (Agaricaceae) is one of the most rare species in the Calvatia genus. It was first validated in 2008. Due to its scarcity, C. nipponica has never been chemically investigated. Its recent discovery in Korea allowed to conduct this study, and using bioactivity-guided fractionation for antiangiogenic activity, chemical investigation of the MeOH extract of the fruiting bodies of C. nipponica led to the isolation of five alkaloids (1 –5 ) and two phenolic compounds (6 –7 ). This is the first study to report the chemical investigation of C. nipponica, and compound1 was previously reported as chemically synthesized only until our report of its isolation and identification from natural sources. The structure of1 was determined by spectroscopic analysis by 1D and 2D NMR spectra and HR-MS. All compounds (1 –7 ) were tested for inhibition of angiogenesis using human umbilical vein endothelial cells (HUVECs). Compounds2, 4 and5 significantly inhibited the promotion of angiogenesis in HUVECs. Compound5 showed the most potent inhibition via downregulation of VEGF, p38 and ERK signaling pathways. These results suggested that the rare mushroom C. nipponica might be beneficial in antiangiogenesis therapy for cancer treatment.