One-pot ligation strategy for atypical ubiquitin chains synthesis by using the trifluoroacetamidomethyl-protected isopeptide-linked Ub (Tfacm-isoUb) unit. Issue 3 (17th January 2018)

• Record Type: Journal Article
• Authors: Si, Yan-Yan; Liang, Lu-Jun; Tang, Shan; Qi, Yun-Kun; Huang, Yong; Zheng, Ji-Shen
• Is Part Of: Tetrahedron letters. Volume 59:Issue 3(2018)
• Page Start: 268
• Page End: 271
• Publication Date: 2018-01-17
• Subjects: Atypical ubiquitin chains -- One-pot native chemical ligation -- Isopeptide-linked Ub (isoUb) -- Trifluoroacetamidomethyl group; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tetlet.2017.12.028
• Languages: English
• ISSNs: 0040-4039

Abstract

Graphical abstract: Highlights: A new one-pot ligation approach was developed for the synthesis of atypical Ub chains based on the Tfacm-isoUb building block. The key intermidate Tfacm-isoUb building block can be readily prepared by Fmoc SPPS. The practicality and efficiency of the new one-pot method is demonstrated by the successful preparation of K11-linked di-Ub. Abstract: Protein chemical synthesis can provide the homogeneous atypical ubiquitin chains that are usually difficult to obtain by other methods. Herein, we report a new one-pot ligation approach for the synthesis of atypical Ub chains based on the Tfacm-protected isoUb building block with operational simplicity and high efficiency. The key intermediate, the Tfacm-protected isoUb building block can be readily prepared by Fmoc SPPS. The practicality and efficiency of the new method is demonstrated by the successful preparation of K11-linked di-Ub.