20(R)-Ginsenoside Rg3 protects SH-SY5Y cells against apoptosis induced by oxygen and glucose deprivation/reperfusion. Issue 16 (15th August 2017)

• Record Type: Journal Article
• Authors: He, Bo; Chen, Peng; Xie, Yu; Li, Shude; Zhang, Xiaochao; Yang, Renhua; Wang, Guihua; Shen, Zhiqiang; Wang, Hui
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 27:Issue 16(2017)
• Page Start: 3867
• Page End: 3871
• Publication Date: 2017-08-15
• Subjects: 20(R)-Ginsenoside Rg3 -- Oxygen-glucose deprivation/reperfusion -- Apoptosis -- Bax -- Bcl-2; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2017.06.045
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: As shown in our previous studies, 20( R )-ginsenoside Rg3 [20( R )-Rg3] exerts a neuroprotective effect on a rat model of transient focal cerebral ischemia, and the mechanism through which it decreases the mRNA expression of calpain I and caspase-3 has been delineated. However, researchers do not know whether 20( R )-Rg3 exhibits a neuroprotective effect following oxygen-glucose deprivation and reperfusion (OGD/R) injury in vitro . In the present study, 20( R )-Rg3 increased cell viability, decreased the LDH leakage rate, and inhibited the apoptosis rate in a concentration-dependent manner. In addition, 20( R )-Rg3 markedly decreased cleaved caspase-3 protein expression. Furthermore, 20( R )-Rg3 significantly decreased the Bax mRNA and protein levels and increased the levels of Bcl-2 mRNA and protein, subsequently decreasing the Bax/Bcl-2 protein ratio. Based on these findings, 20( R )-Rg3 exerts a neuroprotective effect against OGD/R-induced apoptosis.