Isolation of an indigenous Chlorella vulgaris from swine wastewater and characterization of its nutrient removal ability in undiluted sewage. (November 2017)

• Record Type: Journal Article
• Authors: Wen, Yangmin; He, Yongjin; Ji, Xiaowei; Li, Shaofeng; Chen, Ling; Zhou, Youcai; Wang, Mingzi; Chen, Bilian
• Is Part Of: Bioresource technology. Volume 243(2017)
• Page Start: 247
• Page End: 253
• Publication Date: 2017-11
• Subjects: Microalgae -- Chlorella vulgaris -- Swine wastewater -- Nutrient removal -- DNA methylation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.06.094
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: An indigenous microalgal strain was isolated and identified as Chlorella vulgaris . The isolated microalgae could grow well in undiluted swine slurry. TN and TP removal ratios of isolated microalgae were over 90% in swine slurry. The level of 5-mC was inversely proportional to the growth of isolated microalgae. Abstract: Bio-treatment of wastewater mediated by microalgae is considered as a promising solution. This work aimed to isolate an indigenous microalgal strain (named MBFJNU-1) from swine wastewater effluent and identify as Chlorella vulgaris . After 12 days, the removal efficiencies of total nitrogen (TN) and total phosphorus (TP) in undiluted swine slurry were 90.51% and 91.54%, respectively. Stress tolerance in response to wastewater was verified by cultivating in artificial wastewater containing different levels of chemical oxygen demand (COD), TN and TP. MBFJNU-1 could grow well in undiluted swine slurry and artificial wastewater containing 30, 000 mg/L COD or 2000 mg/L TN. Furthermore, global nuclear DNA methylation (5-mC) of MBFJNU-1 was employed to explore the possible mechanism in response to wastewater stress. The results showed that the level of 5-mC was inversely proportional to the growth of MBFJNU-1 in different diluted swine slurry, helping to understand 5-mC variation in response to stress environment.