A xylanase-aided enzymatic pretreatment facilitates cellulose nanofibrillation. (November 2017)

• Record Type: Journal Article
• Authors: Long, Lingfeng; Tian, Dong; Hu, Jinguang; Wang, Fei; Saddler, Jack
• Is Part Of: Bioresource technology. Volume 243(2017)
• Page Start: 898
• Page End: 904
• Publication Date: 2017-11
• Subjects: Xylanase -- Endoglucanase -- Enzymatic pretreatment -- Nanofibrillated cellulose -- Enzyme synergism -- Kraft pulp; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.07.037
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Xylanase-aided endoglucanase pretreatment improved cellulose nanofibrillation. Synergism between endoglucanase and xylanase greatly opened up cellulosic fibers. Endoglucanase-xylanase synergistic cooperation was substrate dependent. Hardwood pulp and xylanase with less β-xylosidase activity were more suitable. This work will open the door for formulating more efficient enzyme cocktails. Abstract: Although biological pretreatment of cellulosic fiber based on endoglucanases has shown some promise to facilitate cellulose nanofibrillation, its efficacy is still limited. In this study, a xylanase-aided endoglucanase pretreatment was assessed on the bleached hardwood and softwood Kraft pulps to facilitate the downstream cellulose nanofibrillation. Four commercial xylanase preparations were compared and the changes of major fiber physicochemical characteristics such as cellulose/hemicellulose content, gross fiber properties, fiber morphologies, cellulose accessibility/degree of polymerization (DP)/crystallinity were systematically evaluated before and after enzymatic pretreatment. It showed that the synergistic cooperation between endoglucanase and certain xylanase (Biobrite) could efficiently "open up" the hardwood Kraft pulp with limited carbohydrates degradation (<7%), which greatly facilitated the downstream cellulose nanofibrillation during mild sonication process (90 Wh) with more uniform disintegrated nanofibril products (50–150 nm, as assessed by scanning electron microscopy and UV–vis spectroscopy).