An efficient production of high-pure xylooligosaccharides from corncob with affinity adsorption-enzymatic reaction integrated approach. (October 2017)

• Record Type: Journal Article
• Authors: Chang, Siyuan; Chu, Jianlin; Guo, Yalan; Li, Huo; Wu, Bin; He, Bingfang
• Is Part Of: Bioresource technology. Volume 241(2017)
• Page Start: 1043
• Page End: 1049
• Publication Date: 2017-10
• Subjects: Xylooligosaccharides -- Adsorption-enzymatic integrated reaction -- Fusion enzyme -- High purity; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.06.002
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Fusion enzyme exhibit a high activity and expression level than native xylanase. Fusion enzyme affinity adsorbed on the corncob substrate. The yield of XOS was 6.91 mg/mL through fusion enzyme hydrolysis. XOS with high purity of 91.7% was obtained by centrifugation. Abstract: Xylooligosaccharides (XOS) are high value-added ingredients for functional foods and they have potential use as prebiotics. In order to reduce the production cost of XOS, we constructed a fusion enzyme consisting of expansin and endo -xylanase from B. subtilis Lucky9 to produce high-pure XOS with affinity adsorption-enzymatic reaction integrated approach. By optimization of inserting linker, the specific xylanase activity of fusion enzyme with R2 linker was increased 1.28-fold than that of native xylanase. Electrophoretic pure fusion enzyme was separated from crude enzyme extracts by affinity adsorption on corncob substrate, with 90.3% recovery in optimized conditions. Then, the fusion enzyme-corncob mixture was directly hydrolyzed. The yield of XOS reached 6.91 mg/mL. The production of XOS with high purity of 91.7% was obtained by one step of centrifugation. This affinity adsorption-enzymatic reaction integrated XOS production approach provides a green route to alternative XOS traditional enzymatic production.