Cellulase and xylanase production at pilot scale by solid-state fermentation from coffee husk using specialized consortia: The consistency of the process and the microbial communities involved. (November 2017)

• Record Type: Journal Article
• Authors: Cerda, Alejandra; Mejías, Laura; Gea, Teresa; Sánchez, Antoni
• Is Part Of: Bioresource technology. Volume 243(2017)
• Page Start: 1059
• Page End: 1068
• Publication Date: 2017-11
• Subjects: Cellulase -- Coffee husk -- Specialized inoculum -- Solid-state fermentation -- Xylanase; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.07.076
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Cellulase and xylanase production by SSF from coffee husk was systematically studied. A specialized inoculum was used to assess the reproducibility of enzyme production. Pilot plant triplicates (50 L) revealed a high reproducibility in process evolution. Gaseous emissions were low, except for volatile organic compounds. A complete microbial characterization of SSF is presented for bacteria and fungi. Abstract: Solid state fermentation is a promising technology however rising concerns related to scale up and reproducibility in a productive process. Coffee husk and a specialized inoculum were used in a 4.5 L and then in 50 L reactors to assess the reproducibility of a cellulase and hemicellulase production system. Fermentations were consistent in terms of cellulase production and microbial communities. The higher temperatures achieved when operating at 50 L generated a shift on the microbial communities and a reduction of nearly 50% on cellulase production at pilot scale. In spite, an overall enzymatic production of 3.1 ± 0.5 FPU g −1 DM and 48 ± 4 U g −1 DM for FPase and Xyl activities was obtained, respectively, with low deviation coefficients of 16 and 19% for FPase and Xyl production. Gaseous emissions assessment revealed an emission factor of 2.6 · 10 −3 kg volatile organic compounds per Mg of coffee husk and negligible NH3, CH4 and N2 O emissions.