Physicochemical and in vitro cytotoxic properties of chitosan from mushroom species (Boletus bovinus and Laccaria laccata). (1st October 2019)

• Record Type: Journal Article
• Authors: Oberemko, Alona; Salaberria, Asier M.; Saule, Rita; Saulis, Gintautas; Kaya, Murat; Labidi, Jalel; Baublys, Vykintas
• Is Part Of: Carbohydrate polymers. Volume 221(2019)
• Page Start: 1
• Page End: 9
• Publication Date: 2019-10-01
• Subjects: Mushroom -- Chitosan -- Cancer -- Low-Molecular weight -- Deacetylation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.05.073
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Chitosan was obtained from two mushroom species ( Boletus bovinus, Laccaria laccata ). Chitosan with low-molecular weight with high degree of deacetylation was obtained. The chitosan exhibited cytotoxic activity against hepatoma and ovary cells. Obtained results may be useful for further investigations of natural anticancer products. Abstract: Chitosan samples from two mushroom species ( Boletus bovinus, Laccaria laccata ) were obtained and characterized by viscosimetry, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), elemental analyses (EA), nuclear magnetic resonance spectroscopy ( 13 C NMR), X-ray diffraction (XRD) and thermogravimetric (TGA) analyses. Properties of the fungal chitosan samples were compared to commercial low-molecular weight chitosan, crustacean chitosan ( Cervimunida johni ) and chitosan obtained from an insect ( Hilobius abietis ). Additionally, the cytotoxic properties of chitosan in vitro on cancerous hepatoma and non-cancerous ovary cells cultivated on films with different chitosan concentrations was evaluated. As a conclusion, this study clearly revealed that low-molecular weight chitosan films and solutions with high degree of deacetylation can act cytotoxically on both tumor MH-22A and normal CHO cells in vitro . Consequently, this work may be useful for further investigations of natural anticancer products in medical areas.