ATG10 (autophagy-related 10) regulates the formation of autophagosome in the anti-virus immune response of pacific oyster (Crassostrea gigas). Issue 91 (August 2019)

• Record Type: Journal Article
• Authors: Han, Zirong; Wang, Weilin; Lv, Xiaojing; Zong, Yanan; Liu, Shujing; Liu, Zhaoqun; Wang, Lingling; Song, Linsheng
• Is Part Of: Fish & shellfish immunology. Issue 91(2019)
• Page Start: 325
• Page End: 332
• Publication Date: 2019-08
• Subjects: Autophagy -- ATG10 -- Autophagosome -- Crassostrea gigas -- Anti-virus immunity; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.05.027
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Autophagy, a highly conserved intracellular degradation system, is involved in numerous processes in vertebrate and invertebrate, such as cell survival, ageing, and immune responses. However, the detailed molecular mechanism of autophagy and its immune regulatory role in bivalves are still not well understood. In the present study, an autophagy-related protein ATG10 (designated as Cg ATG10) was identified from Pacific oyster Crassostrea gigas . The open reading frame of Cg ATG10 cDNA was of 621 bp, encoding a polypeptide of 206 amino acid residues with an Autophagy_act_C domain (from 96 to 123 amino acid), which shared high homology with that from C. virginica and Octopus bimaculoides . The mRNA transcripts of Cg ATG10 were widely expressed in all the tested tissues including mantle, gonad, gills, hemocytes and hepatopancreas, with the highest expression level in mantle. After the stimulation with poly (I:C), the mRNA expression level of Cg ATG10 in the mantle of oysters was significantly up-regulated (4.92-fold of that in Blank group, p < 0.05), and the LC3-conversion from LC3-I to LC3-II (LC3-II/LC3-I) also increased. After an additional injection of dsRNA to knock-down the expression of Cg ATG10 (0.33-fold and 0.10-fold compared respectively with Blank group and dsGFP group, p < 0.05), the downstream conversion of Cg LC3 was inhibited significantly compared with that of the control dsGFP group, while the expression level of autophagy-initiator Cg Beclin1 did not change significantly. In addition, the mRNA transcripts of interferon regulatory factor Cg IRF-1 increased significantly in Cg ATG10-knockdown oysters at 12 h post poly (I:C) stimulation. All the results indicated that Cg ATG10 might participate in the immune response against poly (I:C) by regulating autophagosome formation and interferon system in oysters. Highlights: Cg ATG10 was identified from oyster C. gigas, which was highly expressed in mantle tissue. The mRNA expression of Cg ATG10 in the mantle of oysters was significantly up-regulated after poly (I:C) stimulation. The formation of autophagosome and Cg IRF-1 expression were inhibited in Cg ATG10-knockdown oysters. Cg ATG10 was involved in the formation of autophagosome and anti-virus immune response of oyster.