Anti-inflammatory and wound healing effects of gel containing Kaempferia marginata extract. (10th August 2019)

• Record Type: Journal Article
• Authors: Muthachan, Thawiwan; Tewtrakul, Supinya
• Is Part Of: Journal of ethnopharmacology. Volume 240(2019)
• Publication Date: 2019-08-10
• Subjects: Kaempferia marginata -- Gel -- Anti-inflammatory activity -- Wound healing effect; NO nitric oxide -- LPS lipopolysaccharide -- TNF-α tumor necrosis factor-alpha -- HDF human dermal fibroblast -- RPMI Roswell park memorial institute 1640 medium -- FBS fetal bovine serum -- LPS lipopolysaccharide -- PBS phosphate buffered saline -- DMSO dimethyl sulfoxide -- DMEM dulbecco's modified eagle medium -- HPLC high performance liquid chromatography; 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) -- Griess reagent -- Diclofenac gel; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2019.111964
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Kaempferia marginata Carey (Zingiberaceae family) has been traditionally used in the treatment of inflammation. The whole plant decoction is used for treatment of fever. Ethanol extracts which exert potent anti-inflammatory properties are selected for wound healing assay. Aim of the study: This study aimed to investigate activities of the extract and gel formulation on anti-inflammatory and wound healing activities. The anti-inflammatory and wound healing properties from this plant could support its traditional uses and obtain a new pharmaceutical product with good physical, chemical and biological stabilities. Materials and methods: The anti-inflammatory activity was tested on anti-nitric oxide (NO) production using RAW264.7 cells and wound healing assay using human dermal fibroblast (HDF) cells. Results: The results found that the anti-inflammatory activity of gel containing K. marginata at 10% w/w showed the highest activity with an IC50 value of 12.50 μg/ml (Diclofenac gel, IC50 value = 64.90 μg/ml). It also revealed that wound healing activities of K. marginata gel (5% w/w) showed the highest % cell viability (134.05%) and the highest % cell migration at 85.20 using HDF cells. Conclusion: The present study shows that gel containing K. marginata extracts have good anti-inflammatory and wound healing properties in vitro . Graphical abstract: Image 1