Effects of dietary Gelsemium elegans alkaloids on growth performance, immune responses and disease resistance of Megalobrama amblycephala. Issue 91 (August 2019)

• Record Type: Journal Article
• Authors: Ye, Qiao; Feng, Yongyong; Wang, Zhenlu; Zhou, Aiguo; Xie, Shaolin; Zhang, Yue; Xiang, Qiong; Song, Enfeng; Zou, Jixing
• Is Part Of: Fish & shellfish immunology. Issue 91(2019)
• Page Start: 29
• Page End: 39
• Publication Date: 2019-08
• Subjects: G. elegans alkaloids -- Megalobrama amblycephala -- Growth performance -- Plasma biochemical indices -- Immune responses -- Aeromonas hydrophila; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.05.026
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The present study aim to investigate the effects of dietary Gelsemium elegans alkaloids supplementation in Megalobrama amblycephala . A basal diet supplemented with 0, 5, 10, 20 and 40 mg/kg G . elegans alkaloids were fed to M. amblycephala for 12 weeks. The study indicated that dietary 20 mg/kg and 40 mg/kg G . elegans alkaloids supplementation could significantly improve final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) ( P < 0.05). The 20 mg/kg and 40 mg/kg G . elegans alkaloids groups showed significantly higher whole body and muscle crude protein and crude lipid contents compared to the control group ( P < 0.05). The amino acid contents in muscle were also significantly increased in 20 mg/kg and 40 mg/kg groups ( P < 0.05). Dietary 40 mg/kg G . elegans alkaloids had a significant effect on the contents of LDH, AST, ALT, ALP, TG, TC, LDL-C, HDL-C, ALB and TP in M. amblycephala ( P < 0.05 ). Fish fed 20 mg/kg and 40 mg/kg dietary G. elegans alkaloids showed significant increase in complement 3, complement 4 and immunoglobulin M contents. The liver antioxidant enzymes (SOD, CAT and T-AOC) and MDA content significantly increased at 20 mg/kg and 40 mg/kg G . elegans alkaloids supplement ( P < 0.05). The mRNA levels of immune-related genes IL-1β, IL8, TNF-α and IFN-α were significantly up-regulated, whereas TGF-β and IL10 genes were significantly down-regulated in the liver, spleen and head kidney of fish fed dietary supplementation with 20 mg/kg and 40 mg/kg G . elegans alkaloids. After challenge with Aeromonas hydrophila, significant higher survival rate was observed at 20 mg/kg and 40 mg/kg G . elegans alkaloids supplement ( P < 0.05). Therefore, these results indicated that M. amblycephala fed a diet supplemented with 20 mg/kg and 40 mg/kg G . elegans alkaloids could significantly promote its growth performance, lipids and amino acids deposition, immune ability and resistance to Aeromonas hydrophila . Highlights: Dietary 20 mg/kg and 40 mg/kg G . elegans alkaloids significantly improved growth performance in M. amblycephala. Dietary G. elegans alkaloids significantly increased lipid and amino acid deposition in M. amblycephala. Dietary G. elegans alkaloids significantly enhanced the immunity of M. amblycephala. Dietary G. elegans alkaloids significantly improved the fish survival rate against A. hydrophila. The optimum supplementation level of G. elegans alkaloids in diet was 40 mg/kg.