Biological Imaging with Medium‐Sensitive Bichromatic Flexible Fluorescent Dyes. Issue 49 (8th November 2017)

• Record Type: Journal Article
• Authors: Cao, Yang; Wang, Xiaozhu; Shi, Xiaolei; Clee, Susanne M.; McGeer, Patrick L.; Wolf, Michael O.; Orvig, Chris
• Is Part Of: Angewandte Chemie international edition. Volume 56:Issue 49(2017)
• Page Start: 15603
• Page End: 15606
• Publication Date: 2017-11-08
• Subjects: adipocytes -- bichromatic dyes -- lipophilicity -- myelin sheath -- neurons; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201708529
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: A family of environment‐sensitive shape‐shifting molecules have been developed as flexible fluorescent (FlexFluor) dyes for biological imaging applications. These compounds feature a flexible bithiophene‐based fluorophore that gives rise to different emission colors in lipophilic or hydrophilic environments, as well as side groups that can be synthetically modified with ease. FlexFluor dyes are the first fluorescent dyes in which emission color can be used to indicate lipid/water environments. The behavior of these dyes in different solvents was studied, and used to simultaneously highlight lipid and water contents in adipose and brain tissues using optical fluorescence microscopy. Abstract : Dye Another Day : Environment‐sensitive shape‐shifting molecules function as flexible fluorescent (FlexFluor) dyes for biological imaging applications. The color of light emitted from FlexFluor dyes is dependent on the lipid/water content of the environment. Optical fluorescence microscopy can be used to detect simultaneous emissions in adipose and brain tissues containing lipid and water.