On the Stability of DNA Origami Nanostructures in Low‐Magnesium Buffers. (19th June 2018)

• Record Type: Journal Article
• Authors: Kielar, Charlotte; Xin, Yang; Shen, Boxuan; Kostiainen, Mauri A.; Grundmeier, Guido; Linko, Veikko; Keller, Adrian
• Is Part Of: Angewandte Chemie. Volume 130:Number 30(2018)
• Page Start: 9614
• Page End: 9618
• Publication Date: 2018-06-19
• Subjects: DNA-Nanotechnologie -- DNA-Origami -- Magnesium -- Rasterkraftmikroskopie -- Stabilität; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201802890
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: DNA origami structures have great potential as functional platforms in various biomedical applications. Many applications, however, are incompatible with the high Mg 2+ concentrations commonly believed to be a prerequisite for maintaining DNA origami integrity. Herein, we investigate DNA origami stability in low‐Mg 2+ buffers. DNA origami stability is found to crucially depend on the availability of residual Mg 2+ ions for screening electrostatic repulsion. The presence of EDTA and phosphate ions may thus facilitate DNA origami denaturation by displacing Mg 2+ ions from the DNA backbone and reducing the strength of the Mg 2+ –DNA interaction, respectively. Most remarkably, these buffer dependencies are affected by DNA origami superstructure. However, by rationally selecting buffer components and considering superstructure‐dependent effects, the structural integrity of a given DNA origami nanostructure can be maintained in conventional buffers even at Mg 2+ concentrations in the low‐micromolar range.