Evaluation of IGK and IGL molecular gene rearrangements according to the BIOMED-2 protocols for clinical diagnosis of Hodgkin lymphoma. Issue 3 (15th March 2016)

• Record Type: Journal Article
• Authors: Ghorbian, Saeid; Jahanzad, Issa; Javadi, Gholam Reza; Sakhinia, Ebrahim
• Is Part Of: Hematology. Volume 21:Issue 3(2016)
• Page Start: 133
• Page End: 137
• Publication Date: 2016-03-15
• Subjects: Hodgkin's lymphoma -- BIOMED-2 -- IGK -- Rearrangements -- Clonality; Blood -- Diseases -- Periodicals; Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; 616.15005
• DOI: 10.1179/1607845415Y.0000000035
• Languages: English
• ISSNs: 1024-5332

Abstract

Abstract : Background : Although the analysis of molecular clonality rearrangements of the immunoglobulin light chains ( IGK and IGL ) is an alternative approach for diagnosis of B cell non-Hodgkin lymphomas (NHLs) using BIOMED-2 protocols, NHLs have not been extensively confirmed for Hodgkin lymphoma (HL) cases. We evaluated BIOMED-2 protocols in HL cases, which have been suggested previously as gold standard method for molecular clonality analysis on formalin fixed, paraffin-embedded (FFPE) tissue in NHL patients. Methods : We recruited 50 consecutive FFPE tissues of HL samples to evaluate IGK and IGL clonality gene rearrangements using BIOMED-2 and Heteroduplex methods. Results : Our findings revealed a total of 94% (47/50) positive clonality, which consisted of 70% (35/50) for IGK and 44% (22/50) for IGL . In three cases, clonality was not detected in any of the immunoglobulin gene segments. Conclusions : Analysis of clonality gene rearrangements in IGK and IGL genes using BIOMED-2 protocols could be implemented as a valuable method for improving clonality detection rate in HL cases and sensitivity (94%) and accuracy of HL diagnosis similar to that of the NHL samples will be increased.