Flow cytometric analysis of patients with hereditary spherocytosis – an Indian scenario. Issue 3 (16th March 2018)

• Record Type: Journal Article
• Authors: Manivannan, Prabhu; Tyagi, Seema; Chandra, Dinesh; Mishra, Pravas; Pati, Hara Prasad; Saxena, Renu
• Is Part Of: Hematology. Volume 23:Issue 3(2018)
• Page Start: 175
• Page End: 180
• Publication Date: 2018-03-16
• Subjects: Hereditary spherocytosis -- flow cytometry -- osmotic fragility test -- residual red cells; Blood -- Diseases -- Periodicals; Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; 616.15005
• DOI: 10.1080/10245332.2017.1376855
• Languages: English
• ISSNs: 1024-5332

Abstract

ABSTRACT: Objectives: Flow cytometry osmotic fragility test (FC-OFT) was a recently introduced screening test for hereditary spherocytosis (HS). This study was conducted to evaluate the utility of FC-OFT in all newly diagnosed cases of HS, to compare its diagnostic value with conventional OFT and to correlate with clinical disease severity. Methods: In this study, the percentage of residual red cells (%RRC) was measured using flow cytometer after creating a red cell suspension. Subsequently, this was spiked with deionized water for FC-OFT in all cases of HS ( n = 40), healthy subjects ( n = 40) and beta-thalassemia traits (BTT) ( n = 20). Results: The receiver operator curve analysis defined the optimal cut-offs for FC-OFT-derived indices, such as %RRC value (≤16.29%) and %RRC ratio (>1.72), for HS cases when compared with healthy subjects and BTT ( p < 0.05). The FC-OFT (96%) achieved higher test efficiency than the conventional OF test (68.9%). A significant positive and a negative correlation were found between number of spherocytes/hpf and %RRC ratio ( p = 0.001) and %RRC values ( p = 0.0486). No significant correlation was observed between %RRC value ( p = 0.8934), %RRC ratio ( p = 0.6348) and HS disease severity score. Conclusion: Our results suggest that FC-OFT could be the better screening test for HS cases in developing countries if flow cytometer is available.