Structural and functional characterization of peptide YY on feeding in Schizothorax davidi. Issue 2 (31st May 2018)

• Record Type: Journal Article
• Authors: Zhou, Chaowei; Lei, Luo; Yuan, Dengyue; Deng, Xingxing; Ye, Hua; Luo, Hui; Fang, Jiayang; Yang, Minmin; Li, Yan; Zhang, Chi; Zhou, Jianshe; Wang, Jian; Zeng, Benhe; Zhu, Chengke; Li, Baohai; Zheng, Zonglin
• Is Part Of: Journal of experimental zoology. Volume 329:Issue 2(2018)
• Page Start: 55
• Page End: 61
• Publication Date: 2018-05-31
• Subjects: cloning -- fasting -- feeding -- PYY -- Schizothorax davidi; Zoology -- Periodicals; Zoology; Animal Population Groups -- physiology; Zoology; Electronic journals; Periodical; Periodicals; 590
• DOI: 10.1002/jez.2166
• Languages: English
• ISSNs: 2471-5646

Abstract

Abstract: Several studies have demonstrated that the neuropeptide peptide YY (PYY) plays an important role in feeding in mammals and fish. However, thus far, the feeding regulation function of PYY in Schizothorax davidi has not been well understood. Here, we identified the full‐length cDNA sequence of PYY in S. davidi for the first time. S. davidi PYY contains 803 bp nucleotides including a 328 bp 3′ untranslated region (UTR), a 181 bp 5′ UTR, and a 294 bp open reading frame encoding a peptide of 97 amino acids. S. davidi PYY expression was observed in almost all tissues, with the highest expression detected in the hypothalamus. PYY mRNA expression in the hypothalamus was significantly elevated after a meal ( P < 0.01), and significantly decreased after fasting ( P < 0.01). PYY expression levels were increased sharply following refeeding after 9 days ( P < 0.01), suggesting that it might function as a satiety factor in S. davidi . GRAPHICAL ABSTRACT: S.davidi, a native cold‐water fish from China, is distributed in the upper reaches of the Yangtze River. The full‐length cDNA sequence of PYY in S. davidi was identified for the first time. Our study is the first example to demonstrate the tissues expression of PYY in S. davidi. PYY is widely expressed, notably in the hypothalamus. PYY may as a satiety factor in S. davidi .