Accurate quantification of CXCR4-using HIV-1 variants by Illumina deep-sequencing. (23rd October 2018)

• Record Type: Journal Article
• Authors: Raymond, Stéphanie; Delaugerre, Constance; Nicot, Florence; Assoumou, Lambert; Lancar, Rémi; Beniguel, Lydie; Izopet, Jacques
• Is Part Of: AIDS. Volume 32:Number 16(2018)
• Publication Date: 2018-10-23
• Subjects: AIDS (Disease) -- Periodicals; Acquired Immunodeficiency Syndrome; AIDS (Disease); Periodicals; Periodicals; 616.9792005
• DOI: 10.1097/QAD.0000000000001976
• Languages: English
• ISSNs: 0269-9370

Abstract

Abstract : N/A: Next-generation sequencing is a sensitive method for determining HIV-1 tropism but there is a lack of data on the quantification of X4 variants. We evaluated MiSeq and 454 GS-Junior platforms for determining HIV-1 tropism and for quantifying X4 variants. Both platforms were 93% concordant for determining HIV-1 tropism and correlated well for determining the proportion of X4 variants (Spearman correlation, ρ = 0.748; P < 0.0001). MiSeq Illumina sequencing seems to be well adapted for characterizing X4-containing samples.