Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection. (24th March 2009)

• Record Type: Journal Article
• Authors: Ayada, Kiyoshi; Yokota, Kenji; Kawahara, Yoshiro; Yamamoto, Yumiko; Hirai, Kazuyuki; Inaba, Tomoki; Kita, Masahide; Okada, Hiroyuki; Yamamoto, Kazuhide; Oguma, Keiji
• Other Names: Matsuura Eiji Academic Editor.
• Is Part Of: Clinical & developmental immunology. Volume 2009(2009)
• Publication Date: 2009-03-24
• Subjects: Developmental immunology -- Periodicals; Clinical immunology -- Periodicals; Immune System -- immunology -- Periodicals; Immune System -- growth & development -- Periodicals; Immune System Diseases -- immunology -- Periodicals; 571.9638
• DOI: 10.1155/2009/850623
• Languages: English
• ISSNs: 1740-2522

Abstract

Abstract : Helicobacter pylori ( H. pylori ) infection is a definite causative factor for gastric ulcers (GUs). In the present study we detected a specific antigen of gastric epithelial cells (HGC-27) using cell ELISA, which was recognized by the sera of GU patients (n = 20 ) but not in patients with chronic gastritis (CG;n = 20 ) or in healthy volunteers (HC;n = 10 ). This antigen was over-expressed by a stressful (heat-stressed) environment, and was identified as elongation factor 2 kinase (EF-2K) by western blotting. The GU patients' lymphocytes stimulated by H. pylori specifically disrupted heat-stressed HGC-27 cells in a cytotoxic assay. In flow cytometry, the effector cells (lymphocytes) from GU patients were significantly differentiated to T helper type 1 lymphocyte (Th1) and cytotoxic T lymphocyte (CTL) as opposed to those from CG patients. The target cells (HGC-27) expressed EF-2K and MHC-class I together with costimulatory molecules from heat stress. This antigen specific immune mechanism could have a prominent role in the pathogenesis of GU.