Clarification and 3-D visualization of immunolabeled human placenta villi. (May 2017)

• Record Type: Journal Article
• Authors: Merz, George; Schwenk, Valerie; Shah, Ruchit G.; Necaise, Phillip; Salafia, Carolyn M.
• Is Part Of: Placenta. Volume 53(2017:May)
• Page Start: 36
• Page End: 39
• Publication Date: 2017-05
• Subjects: Placenta -- Tissue clarification -- Confocal microscopy -- Immunofluorescence -- Capillary network; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2017.03.015
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: We report here the successful 3D visualization of human placenta villous structures on the order of ∼1 mm 3 by a combination of immunolabeling, rapid tissue clarification and laser scanning confocal microscopy. The resultant image sets exhibit a complex arrangement of villi and their contained vasculature that mirrors their arrangement in situ . Highlights: A rapid and simplified method to immunolabel and clarify placental tissue. Successful confocal microscopic imaging of large volumes (∼1 mm 3 ) of villus trees. 3D visualization of villi and their capillaries across gestational ages.