The interactions of fullerene C60 and Benzo(α)pyrene influence their bioavailability and toxicity to zebrafish embryos. (October 2018)

• Record Type: Journal Article
• Authors: Della Torre, Camilla; Maggioni, Daniela; Ghilardi, Anna; Parolini, Marco; Santo, Nadia; Landi, Claudia; Madaschi, Laura; Magni, Stefano; Tasselli, Stefano; Ascagni, Miriam; Bini, Luca; La Porta, Caterina; Del Giacco, Luca; Binelli, Andrea
• Is Part Of: Environmental pollution. Volume 241(2018)
• Page Start: 999
• Page End: 1008
• Publication Date: 2018-10
• Subjects: Fullerene nanoparticles -- Danio rerio -- Oxidative stress -- Proteomics -- Trojan horse effect; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2018.06.042
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: This study aimed to assess the toxicological consequences related to the interaction of fullerene nanoparticles (C60 ) and Benzo(α)pyrene (B(α)P) on zebrafish embryos, which were exposed to C60 and B(α)P alone and to C60 doped with B(α)P. The uptake of pollutants into their tissues and intra-cellular localization were investigated by immunofluorescence and electron microscopy. A set of biomarkers of genotoxicity and oxidative stress, as well as functional proteomics analysis were applied to assess the toxic effects due to C60 interaction with B(α)P. The carrier role of C60 for B(α)P was observed, however adsorption on C60 did not affect the accumulation and localization of B(α)P in the embryos. Instead, C60 doped with B(α)P resulted more prone to sedimentation and less bioavailable for the embryos compared to C60 alone. As for toxicity, our results suggested that C60 alone elicited oxidative stress in embryos and a down-regulation of proteins involved in energetic metabolism. The C60 + B(α)P induced cellular response mechanisms similar to B(α)P alone, but generating greater cellular damages in the exposed embryos. Graphical abstract: Highlights: Adsorption of B(α)P increases C60 sedimentation process. The B(α)P adsorbed on C60 is accumulated in embryos similarly to B(α)P alone. C60 exposure triggers oxidative stress condition. B(α)P alone and adsorbed on C60 induced the expression of stress proteins. C60 doped with B(α)P produced greater cellular effect. Abstract : Once C60 nanoparticles and B(α)P meet in water, they reciprocally affect their bioavailability and, by consequence, their toxicity to organisms.