Cousins at work: How combining medical with optical imaging enhances in vivo cell tracking. (September 2018)

• Record Type: Journal Article
• Authors: Volpe, Alessia; Kurtys, Ewelina; Fruhwirth, Gilbert O.
• Is Part Of: International journal of biochemistry & cell biology. Volume 102(2018)
• Page Start: 40
• Page End: 50
• Publication Date: 2018-09
• Subjects: BLI bioluminescence imaging -- CEST chemical exchange saturation transfer -- CLI Cerenkov luminescence imaging -- CM confocal fluorescence microscopy -- CT X-ray computed tomography -- FLI/FRI fluorescence imaging/fluorescence reflectance imaging -- FMT fluorescence mediated tomography -- HF high-frequency -- IVM intravital microscopy -- MRI magnetic resonance imaging -- MSOT multispectral optoacoustic tomography -- NIR near-infrared spectrum -- OCT optical coherence tomography -- OPT optical projection tomography -- PAT photoacoustic tomography -- RSOM high-resolution raster scanning optoacoustic mesoscopy -- PET positron emission tomography -- SPECT single photon computed emission tomography -- SRM super-resolution microscopy (a group of various technologies including but not limited to photoactivated localisation microscopy (PALM), various stochastic optical reconstruction microscopy (STORM) techniques, stimulated emission depletion microscopy (STED), and ground state depletion individual molecule return (GSDIM)) -- TPM tow-photon excitation microscopy -- US ultrasound imaging including Doppler and high-frequency ultrasound techniques -- VIS visible light spectrum; Cancer metastasis -- Cell therapy -- Microscopy -- Reporter genes -- Whole-body imaging; Biochemistry -- Periodicals; Cytology -- Periodicals; Biochemistry -- Periodicals; Cell Biology -- Periodicals; Biochimie -- Périodiques; Cytologie -- Périodiques; Biochimie; Cytologie; Biochemistry; Cytology; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); Periodicals; 572.05
• DOI: 10.1016/j.biocel.2018.06.008
• Languages: English
• ISSNs: 1357-2725

Abstract

Abstract: Microscopy and medical imaging are related in their exploitation of electromagnetic waves, but were developed to satisfy differing needs, namely to observe small objects or to look inside subjects/objects, respectively. Together, these techniques can help elucidate complex biological processes and better understand health and disease. A current major challenge is to delineate mechanisms governing cell migration and tissue invasion in organismal development, the immune system and in human diseases such as cancer where the spatiotemporal tracking of small cell numbers in live animal models is extremely challenging. Multi-modal multi-scale in vivo cell tracking integrates medical and optical imaging. Fuelled by basic research in cancer biology and cell-based therapeutics, it has been enabled by technological advances providing enhanced resolution, sensitivity and multiplexing capabilities. Here, we review which imaging modalities have been successfully used for in vivo cell tracking and how this challenging task has benefitted from combining macroscopic with microscopic techniques.