BMP-2 plasmid DNA-loaded chitosan films – A new strategy for bone engineering. Issue 12 (December 2017)

• Record Type: Journal Article
• Authors: Li, Juan; Lin, Jun; Yu, Wenke; Song, Xiaojia; Hu, Qiaoling; Xu, Jing-Hong; Wang, Huiming; Mehl, Christian
• Is Part Of: Journal of cranio-maxillofacial surgery. Volume 45:Issue 12(2017)
• Page Start: 2084
• Page End: 2091
• Publication Date: 2017-12
• Subjects: Bone morphogenetic protein 2 -- Chitosan -- Drug delivery systems -- DNA -- Bone -- Histology; Skull -- Surgery -- Periodicals; Maxilla -- Surgery -- Periodicals; Face -- Surgery -- Periodicals; Surgery, Plastic -- Periodicals; Maxilla -- surgery -- Periodicals; Face -- surgery -- Periodicals; Skull -- surgery -- Periodicals; Oral Surgical Procedures -- Periodicals; Reconstructive Surgical Procedures -- Periodicals; Surgery, Plastic -- Periodicals; Surgery, Oral -- Periodicals; Electronic journals; 617.514
• DOI: 10.1016/j.jcms.2017.10.005
• Languages: English
• ISSNs: 1010-5182

Abstract

Abstract: Objectives: Bone defects are common in every area of medicine and remain a clinical challenge. Tissue engineering has led to promising new strategies in accelerating bone repair. Bone morphogenetic proteins (BMPs) play crucial roles in bone regeneration, but are required in supra-physiological doses, which are expensive and produce severe side effects. Methods: To address these issues, we prepared BMP-2 plasmid DNA-loaded chitosan films, and examined their effects on mouse osteoblast-like MC3T3-E1 cell morphology, proliferation, and runt-related transcription factor 2 (RUNX2) expression. In vivo testing was performed using calvarial critical-sized defects and histomorphometry in 36 Sprague–Dawley rats. Unloaded chitosan films and empty defects served as controls. Results: In contrast to the controls, cells grown on BMP-2 plasmid DNA-loaded chitosan films had well established filopodia and lamellipodia, significantly higher proliferation 2, 4, and 6 days post-seeding ( P ≤ 0.05), and higher nuclear RUNX2 expression. In vivo, new bone growth was significantly greater in the BMP-2 group than in the control groups at 4, 8, and 12 weeks ( P ≤ 0.01). Conclusions: Based on our study findings, BMP-2 plasmid DNA-loaded chitosan films provide an effective strategy for GBR, combining cellular compatibility with biocapability in vivo .