Automated radiosynthesis of [18F]FBEM, a sulfhydryl site specific labeling agent for peptides and proteins. (October 2018)

• Record Type: Journal Article
• Authors: Lim, Keunpoong; Ropchan, Jim; Kiesewetter, Dale O.; Chen, Xiaoyuan; Huang, Yiyun
• Is Part Of: Applied radiation and isotopes. Volume 140(2018:Oct.)
• Page Start: 294
• Page End: 299
• Publication Date: 2018-10
• Subjects: [18F]FBEM -- Automation -- Thiol(-SH)-specific -- Radiotracer synthesis -- PET; Radiology -- Periodicals; Radiation -- Industrial applications -- Periodicals; Nuclear chemistry -- Periodicals; Internet resource; Periodical; 660.298
• DOI: 10.1016/j.apradiso.2018.07.033
• Languages: English
• ISSNs: 0969-8043

Abstract

Abstract: In the process of developing [ 18 F]FBEM coupled target peptide, we have instituted a robust automated synthesis of [ 18 F]FBEM, a sulfhydryl (-SH) site specific agent for radiolabeling of peptides and proteins. The radiosynthesis generated 1.67–3.89 GBq (45.1–105.1 mCi, 7.5–18.8% non-decay corrected yield) of [ 18 F]FBEM from 22.2 GBq (600 mCi) of starting [ 18 F]fluoride with molar activity of 31.8 ± 5.3 GBq/µmol (0.86 ± 0.14 mCi/nmol) (n = 3) at the end of synthesis. Radiochemical purity was greater than 98%, and total synthesis time was ~90 min. Highlights: Radiosynthesis of [ 18 F]FBEM was performed in a TRACERlab FXN-Pro automated module. [ 18 F]FBEM was obtained from a 3-step reaction sequence in two separate reactors. Total synthesis time from EOB to the [ 18 F]FBEM was about 90 min. The procedure can be applied in a routine preparation of [ 18 F]FBEM. Non-decay corrected radiochemical yield for [ 18 F]FBEM was 7.5–18.8%.