A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study. (4th December 2013)

• Record Type: Journal Article
• Authors: Jebaliya, Hetal; Patel, Madhavi; Jadeja, Yashwant; Dabhi, Batuk; Shah, Anamik
• Other Names: Takeuchi Toyohide Academic Editor.
• Is Part Of: Chromatography research international. Volume 2013(2013)
• Publication Date: 2013-12-04
• Subjects: Chromatographic analysis -- Periodicals; Chromatographic analysis -- Research -- Periodicals; Chromatographic analysis; Chromatographic analysis -- Research; Periodicals
• DOI: 10.1155/2013/673150
• Languages: English
• ISSNs: 2090-3502

Abstract

Abstract : The simplest stability indicating reversed phase Isocratic HPLC and UPLC methods has been developed and validated for the determination of fluconazole in bulk and solid pharmaceutical dosage form. A SunFire C18 (250 × 4.5 mm, 5 μ m particle size) column has been used for HPLC and BEH C18 (100 × 2.1 mm, 1.7 μ m particle size) column used for UPLC. The Mobile phase consisted of Methanol : Water (70 : 30) for HPLC and Methanol : Water (55 : 45 v/v) for UPLC. Isocratic flow was set at 1 mL/min and 0.30 mL/min, respectively, for HPLC and UPLC. For both HPLC and UPLC system detection has been performed at 211 nm with 30°C column oven temperature (good elution was obtained at 30°C) and injection volume, respectively, 2 μ L and 20 μ L for HPLC and UPLC.