Antibacterial activities of plasma from the common (Chelydra serpentina) and alligator snapping turtle (Macrochelys temminckii). Issue 2 (1st October 2018)

• Record Type: Journal Article
• Authors: Baker, Sarah J.; Kessler, Ethan J.; Merchant, Mark E.
• Is Part Of: Journal of experimental zoology. Volume 331:Issue 2(2019)
• Page Start: 85
• Page End: 92
• Publication Date: 2018-10-01
• Subjects: antimicrobial -- Chelydridae -- innate immunity -- reptile -- serum complement -- turtle; Zoology -- Periodicals; Zoology; Animal Population Groups -- physiology; Zoology; Electronic journals; Periodical; Periodicals; 590
• DOI: 10.1002/jez.2237
• Languages: English
• ISSNs: 2471-5646

Abstract

Abstract: Innate immunity provides a fast‐acting and nonspecific defense against microbial infection, and appears to have particular importance in the immune response of ectothermic vertebrates. Chelonians are a globally distributed and diverse group, yet little is known about their basic immune function. The chelonian family Chelydridae is made up of two genera ( Chelydra and Macrochelys ), represented in our study by the widespread common snapping turtle ( Chelydra serpentina ; CST) and the southeast USA endemic alligator snapping turtle ( Macrochelys temminckii ; AST). Our goal was to quantify the innate immune response of the family Chelydridae, using the antibacterial activity of plasma as a measure of immune function. Our results show that the plasma of both species has strong antibacterial properties, but CST plasma kills a higher percentage of bacteria than AST plasma. In addition, while both species showed the highest antibacterial activity at 25 to 30°C, CST plasma retained its antibacterial properties at lower and higher temperatures than AST plasma. Our results indicate that, like many ectotherms, Chelydridae have a relatively strong innate immune response. The stronger, more robust immune response of CSTs compared with ASTs is likely correlated to the differences in geographic ranges but may also have implications for each species' tolerance to anthropogenic habitat degradation and global climate change. Abstract : Thermal profile of antibacterial activity in common and alligator snapping turtle plasma. HIGHLIGHTS: The plasma of common snapping turtle (CST) and alligator snapping turtle (AST) both exhibit potent, broad‐acting antibacterial activities. The antibacterial activities are rapid, peaking within 20 min of exposure to microbes. The thermal profiles of the antibacterial properties of plasma derived from AST and CST are reflective of their latitudinal distributions. The antibacterial activities are heat labile and inhibited by protease and ethylenediaminetetraacetic acid, which are characteristics of innate immune activity mediated by serum complement.