Detection of hepatitis B surface antigen by immunoassay using magnetite nanoparticles binding hepatitis B surface antibody. Issue 4 (4th July 2019)

• Record Type: Journal Article
• Authors: Thanh, Bui Trung; Hai, Tran Hoang; Van, Pham Hung; Minh Tung, Le; Lee, Jaeryeong
• Is Part Of: Geosystem engineering. Volume 22:Issue 4(2019)
• Page Start: 206
• Page End: 213
• Publication Date: 2019-07-04
• Subjects: Magnetite nanoparticles -- detection -- hepatitis B surface antigen -- hepatitis B surface antibody -- immunoassay; Mining engineering -- Periodicals; Petroleum engineering -- Periodicals; Gas engineering -- Periodicals; Geology, Economic -- Periodicals; 620
• DOI: 10.1080/12269328.2018.1540310
• Languages: English
• ISSNs: 1226-9328

Abstract

ABSTRACT: The detection of hepatitis B surface antigen (HBsAg) with an immunoassay using magnetite nanoparticles (NPs) that bind hepatitis B surface antibody (anti-HBs) was investigated. Magnetite NPs with an average size of 32 nm were synthesized and functionalized using tetraethyl orthosilicate, 3-aminopropyltriethoxysilane, glutaraldehyde, and streptavidin. The functionalized NPs were conjugated with biotinylated anti-HBs to capture HBsAg. HBsAgs were detected with a sensitivity, specificity and agreement of 98%, 97%, and 97.5%, respectively, when compared to the rapid test. In addition, the novel method could also enrich HBsAgs, using different tube sizes and can facilitate amplification for the detection of microorganisms.