A novel foam based separation strategy for extracting minute target impurities. Issue 12 (15th May 2019)

• Record Type: Journal Article
• Authors: Sadegh, Shayan; Dasarathy, Dhweeja; Ito, Yoichiro
• Is Part Of: Journal of separation science. Volume 42:Issue 12(2019)
• Page Start: 2093
• Page End: 2099
• Publication Date: 2019-05-15
• Subjects: dry foam -- dye separation -- foam separation -- high performance liquid chromatography -- separatory bottle; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201900057
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: The purpose of this study was to extract impurities from compounds using a simple separatory bottle to purify target compounds with a foam column and allow for the further characterization of impurities. Charged dyes were used as target compounds due to the ease of detection of dyes and isolated impurities. Foaming agents were used in a glass bottle with a modified cap to separate a target impurity using an appropriately charged ligand. By passing N2 gas through the solution, the surfactants sodium dodecyl sulfate and cetylpyridinium chloride generated foams that separated the dyes, Methylene blue and Orange G, respectively, from a solution containing both dyes. Sodium dodecyl sulfate condensed Methylene blue from the solution with high purity while cetylpyridinium chloride condensed Orange G with less purity. A range of concentrations (0.01–0.5 mmol/L) of dyes were used for separation. The condensability (volume and/or concentration) of the target compound increased as its concentration decreased. This novel separation method is a simple, rapid, inexpensive, and effective way to prepare samples and allows for the characterization of these impurities using sensitive analytical detection techniques.