Predominance of blaCTX-M-65 and blaCTX-M-55 in extended-spectrum β-lactamase-producing Escherichia coli from raw retail chicken in South Korea. (June 2019)

• Record Type: Journal Article
• Authors: Park, Hyeeun; Kim, Jinshil; Ryu, Sangryeol; Jeon, Byeonghwa
• Is Part Of: Journal of global antimicrobial resistance. Volume 17(2019)
• Page Start: 216
• Page End: 220
• Publication Date: 2019-06
• Subjects: Antimicrobial resistance -- Extended-spectrum β-lactamase -- ESBL -- CTX-M -- Escherichia coli -- Chicken; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2019.01.005
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: First characterisation of extended-spectrum β-lactamase-producing E. coli (ESBL-EC) from raw retail chicken in South Korea. bla CTX-M-65 was the predominant ESBL gene in ESBL-EC isolates from chicken. Multidrug resistance is highly common in ESBL-EC from retail chicken in South Korea. ESBL-EC isolates from retail chicken in South Korea possess virulence genes of extraintestinal pathogenic E. coli (ExPEC). Abstract: Objectives: Extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) are a serious public health concern worldwide. The aim of this study was to characterise ESBL-EC isolated from raw retail chicken in South Korea. Methods: The antimicrobial resistance, phylogenetic group and virulence gene prevalence of 67 ESBL-EC isolated from retail chicken in South Korea were investigated. Results: All of the isolates possessed bla CTX-M genes, predominantly bla CTX-M-65 (52.2%) and bla CTX-M-55 (25.4%), and three isolates harboured both bla CTX-M-65 and bla CTX-M-55 . More than one-half of the ESBL-EC strains also carried bla TEM . Antimicrobial susceptibility testing revealed that 98.5% of the strains were multidrug-resistant (MDR). Phylogenetic analysis showed that group A was predominant (56.7%), followed by B1 (19.4%), E (8.9%), B2 (6.0%) and D (6.0%). Virulence genes associated with extraintestinal pathogenic E. coli (ExPEC) were frequently detected in isolates of phylogenetic groups B1, B2, D and E. Conclusion: The results in this study demonstrate that retail chicken in South Korea is highly contaminated with MDR ESBL-EC and may serve as a reservoir for transmitting ExPEC to humans.