A two-photon fluorescent probe for imaging of endogenous formaldehyde in HeLa cells and quantitative detection of basal formaldehyde in milk samples. Issue 23 (28th May 2019)

• Record Type: Journal Article
• Authors: Xin, Fangyun; Tian, Yong; Jing, Jing; Zhang, Xiaoling
• Is Part Of: Analytical methods. Volume 11:Issue 23(2019)
• Page Start: 2969
• Page End: 2975
• Publication Date: 2019-05-28
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c9ay00553f
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A fluorescent probeNaP which can image endogenous formaldehyde in living cells and quantitatively detect basal formaldehyde in milk samples. Abstract : Herein, a two-photon fluorescent probeNaP for formaldehyde (FA) was rationally constructed and reported. The probe presented favorable properties responding to FA, such as a remarkable ∼29-fold fluorescence signal enhancement at 550 nm, good selectivity, high sensitivity and a low detection limit which was calculated to be 1.62 × 10 −6 M. Furthermore, the probeNaP was membrane-permeable and successfully employed for visualization of endogenous FA in living cells. Meanwhile, the FA content in six milk samples was quantitatively detected and the accuracy was assessed by the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method, which is a standard UV spectrophotometric method. As expected, the proposed probeNaP may serve as a promising tool for imaging of endogenous FA in living systems and quantitative detection of FA in the food industry.