CAMP-modulated biomimetic ionic nanochannels based on a smart polymer. Issue 23 (20th May 2019)

• Record Type: Journal Article
• Authors: Chen, Zhixiang; Sun, Taolei; Qing, Guangyan
• Is Part Of: Journal of materials chemistry. Volume 7:Issue 23(2019)
• Page Start: 3710
• Page End: 3715
• Publication Date: 2019-05-20
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c9tb00639g
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Dynamic gating behaviour of ionic nanochannel is precisely manipulated by cyclic 3′, 5′-adenosine monophosphate (cAMP) by taking advantage of reversible conformational transition of the smart polymer chains in response to cAMP specific adsorption, which provides a new idea for developing smart nanochannels regulated by crucial signal-biomolecules. Abstract : The flow of ions across the cell membrane is essential to many life processes. Cyclic 3′, 5′-adenosine monophosphate (cAMP) binds to and regulates the function of cyclic nucleotide-gating channel proteins, which directly mediate the Na + /Ca 2+ transmembrane behaviours. This inspires us to develop a biomimetic cAMP-modulated ionic nanochannel based on a cAMP-responsive polymer design. Driven by specific hydrogen bonding interactions with cAMP, an arginine-based smart copolymer film displays remarkable adsorption toward cAMP and can clearly discriminate cAMP from other nucleotides, accompanied with reversible conformational transition of the polymer chain from a contracted state to a swollen one. Benefiting from these features, dynamic gating behaviours of the nanochannels located on the copolymer-modified anodic aluminum oxide (AAO) membrane could be precisely manipulated by cAMP. Sensitive responsiveness (10 pmol L −1 ) and high specificity toward cAMP, high controllability and satisfactory reversibility reveal the great potential of biomolecule responsive polymers in biomimetic nanochannels and nanodevices.