Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1, 4-galactosyltransferases. Issue 24 (5th June 2019)

• Record Type: Journal Article
• Authors: Huang, Kun; Parmeggiani, Fabio; Ledru, Helene; Hollingsworth, Kristian; Mas Pons, Jordi; Marchesi, Andrea; Both, Peter; Mattey, Ashley P.; Pallister, Edward; Bulmer, Gregory S.; van Munster, Jolanda M.; Turnbull, W. Bruce; Galan, M. Carmen; Flitsch, Sabine L.
• Is Part Of: Organic & biomolecular chemistry. Volume 17:Issue 24(2019)
• Page Start: 5920
• Page End: 5924
• Publication Date: 2019-06-05
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c9ob01089k
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Synthesis of LacNAc with reversible GalTs. Abstract : Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1, 4-galactosyltransferases to catalyse the transgalactosylation from lactose to N -acetylglucosamine to form N -acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of p NP-β-LacNAc from lactose using β1, 4-galactosyltransferase NmLgtB-B as the only biocatalyst.