Green, infrared‐assisted extraction based on statistical modeling for ultra‐high performance liquid chromatography determination of bioactive isoflavones from Puerariae Lobatae. Issue 6 (22nd May 2019)

• Record Type: Journal Article
• Authors: Li, Hua; He, Jingxia; Yin, Rongli; Mou, Qianqian; Bai, Yuqi; Yang, Bin
• Is Part Of: Separation science plus. Volume 2:Issue 6(2019)
• Page Start: 216
• Page End: 224
• Publication Date: 2019-05-22
• Subjects: infrared‐assisted extraction -- isoflavones -- pueraria lobata -- response surface methodology -- ultra high performance liquid chromatography; Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1002/sscp.201900030
• Languages: English
• ISSNs: 2573-1815

Abstract

Abstract: We developed a green infrared‐assisted extraction method for the determination of 3′‐hydroxy puerarin, puerarin, daidzin, genistin, and daidzein in Pueraria lobata . After a simultaneous optimization of Box–Behnken design and desirability function, the developed method provided better isoflavone extraction efficiencies with a lower solvent consumption and shorter extraction time than other methods. The optimized extraction used an extraction time of 8 min, solid–liquid ratio of 1:100, and ethanol volume fraction of 60%. Additionally, ultra‐high performance liquid chromatography was used for identification and quantification of the five isoflavones. Our method showed good linearity ( r >0.999) over wide concentration ranges. The ranges for the limits of detection and quantitation were 0.03–0.09 μg/mL and 0.14–0.44 μg/mL, respectively, the recovery range was 98.38–103.37%, and the run time was 5.5 min. This approach is efficient, economical, and eco‐friendly, and shows promise for rapid extraction and quantitative analysis of bioactive isoflavones from Pueraria lobata .