A new mouse model for noninvasive fluorescence‐based monitoring of mitochondrial UCP1 expression. Issue 11 (20th May 2019)

• Record Type: Journal Article
• Authors: Kawarasaki, Satoko; Kuwata, Hidetoshi; Sawazaki, Honami; Sakamoto, Tomoya; Nitta, Takahiro; Kim, Chuu‐Sook; Jheng, Huei‐Fen; Takahashi, Haruya; Nomura, Wataru; Ara, Takeshi; Takahashi, Nobuyuki; Tomita, Koichi; Yu, Rina; Kawada, Teruo; Goto, Tsuyoshi
• Is Part Of: FEBS letters. Volume 593:Issue 11(2019)
• Page Start: 1201
• Page End: 1212
• Publication Date: 2019-05-20
• Subjects: brown adipose tissue -- cold exposure -- in vivo imaging -- mRFP1 -- UCP1 -- β3‐adrenergic stimulation; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13430
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Abstract : Mitochondrial uncoupling protein 1 (UCP1) is well known for its thermogenic function in brown adipose tissue (BAT). Since UCP1 expends energy on thermogenesis, UCP1 activation has been considered an approach to ameliorate obesity. As a tool for uncovering yet unknown mechanisms of UCP1 activation, we generated a transgenic mouse model in which UCP1 expression levels are reflected in fluorescence derived from monomeric red fluorescent protein 1 (mRFP1). In these UCP1‐mRFP1 BAC transgenic mice, fluorescence intensity mimics the change in UCP1 expression levels evoked through physiological or pharmacological stimulation. This transgenic mouse model will be useful in the search for bioactive compounds with the ability to induce UCP1 and for revealing undiscovered mechanisms of BAT activation.