Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles. Issue 23 (31st January 2019)

• Record Type: Journal Article
• Authors: Wilkins, Laura E.; Hasan, Muhammad; Fayter, Alice E. R.; Biggs, Caroline; Walker, Marc; Gibson, Matthew I.
• Is Part Of: Polymer chemistry. Volume 10:Issue 23(2019)
• Page Start: 2986
• Page End: 2990
• Publication Date: 2019-01-31
• Subjects: Polymers -- Periodicals; Macromolecules -- Periodicals; Polymerization -- Periodicals; 547.705
• DOI: 10.1039/c8py01719k
• Languages: English
• ISSNs: 1759-9954

Abstract

Abstract : Antifreeze proteins are site-specifically conjugated onto polymer-stabilised gold nanoparticles, resulting in hybrid materials capable of modulating ice growth processes. Abstract : Antifreeze proteins (AFPs) have many potential applications, ranging from cryobiology to aerospace, if they can be incorporated into materials. Here, a range of engineered AFP mutants were prepared and site-specifically conjugated onto RAFT polymer-stabilized gold nanoparticles to generate new hybrid multivalent ice growth inhibitors. Only the SNAP-tagged AFPs lead to potent 'antifreeze' active nanomaterials with His-Tag capture resulting in no activity, showing the mode of conjugation is essential. This versatile strategy will enable the development of multivalent AFPs for translational and fundamental studies.